Composite

Part:BBa_K1462120:Design

Designed by: Chuyao Fan   Group: iGEM14_SCUT   (2014-10-10)

Gal1+Hbd+GFP+ADH1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 942
    Illegal BsaI.rc site found at 2054


Design Notes

This part is constructed as a control group to identify our enzymes have been directed to the mitochondria.The GFP is fused at the C-terminus of Hbd to report where the enzyme located.Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins.

Figure 1. Enzymes located in cytoplasm The fluorescence indicates that Hbd is expressed in the cytoplasm